Gotham Under Siege is the East End and Burnley districts of Gotham City transformed into a magical battlezone as the forces of Wonder Woman oppose the forces of Hippolyta/Circe as they converge upon the city without discrimination to destroy Brainiac's remaining forces in Old Gotham.
Wonder Woman's rebel forces are stationed on the roof of the LexCorp Reclamation Project while Hippolyta's loyalist forces are stationed on the roof of a restaurant across from the Gotham Mercy General Hospital.
Gotham City is under assault. An enormous magical barrier has been erected by Hippolyta to contain Brainiac’s forces before the attack begins. Invisible battleships patrol the skies and unload troops and supplies for the assault to come. Creatures from Greek mythology pour into the streets as the warring factions of Amazons begin to form their battle lines. Solo missions in this re-envisioned Gotham City allow players to work with the rebels to defend Gotham City and halt the advance of the loyalists, or take up arms with the queen and prepare to reduce the city to rubble.
- Players travel to an alternate version of Gotham City – Gotham Under Siege. From there, Heroes and Villains will each have access to Daily Solo Missions and Bounty Missions. The bounty mission will direct players to one of four locations within Amazon-controlled Gotham City where they are charged with defeating an iconic boss. Heroes will combat Catwoman, Killer Croc, Mister Freeze, or Cheetah, while Villains will fight Donna Troy, Wonder Girl, Nightwing, or Supergirl.
- Gotham Under Siege is accessible with Amazon Fury Part I or Amazon Fury Part II.
- In addition to Wonder Woman's Rebels and Hippolyta's Loyalists, the Gotham City Police Department can be found combating Amazons in the East End district while members of Mister Freeze's Streetgang can be found combating Amazons at the Gotham Mercy General Hospital.